HIV-1 Adapts To Replicate in Cells Expressing Common Marmoset APOBEC3G and BST2.

ABSTRACT:

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Previous studies have shown that a major block to HIV-1 replication in common marmosets operates at the level of viral entry and that this block can be overcome by adaptation of the virus in tissue-cultured cells. However, our current studies indicate that HIV-1 encounters additional postentry blocks in common marmoset peripheral blood mononuclear cells. Here, we show that the common marmoset APOBEC3G (A3G) and BST2 proteins block HIV-1 in cell cultures. Using a directed-evolution method that takes advantage of the natural ability of HIV-1 to mutate during replication, we have been able to overcome these blocks in tissue-cultured cells. In the adapted viruses, specific changes were observed in gag, vif, env, and nef. The contribution of these changes to virus replication

SUBMITTER: Fernandez-Oliva A 

PROVIDER: S-EPMC4702673 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature