Quantification of decellularized human myocardial matrix: A comparison of six patients.

ABSTRACT:

Purpose

The purpose of this study was to characterize and quantitatively analyze human cardiac extracellular matrix (ECM) isolated from six different cadaveric donor hearts.

Experimental design

ECM was isolated by decellularization of six human cadaveric donor hearts and characterized by quantifying sulfated glycosaminoglycan content (sGAG) and via PAGE. The protein content was then quantified using ECM-targeted Quantitative conCATamers (QconCAT) by LC-SRM analysis using 83 stable isotope labeled (SIL) peptides representing 48 different proteins. Nontargeted global analysis was also implemented using LC-MS/MS.

Results

The sGAG content, PAGE, and QconCAT proteomics analysis showed significant variation between each of the six patient samples. The quantitative proteomic

SUBMITTER: Johnson TD 

PROVIDER: S-EPMC4703489 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature