The bimodally expressed microRNA miR-142 gates exit from pluripotency.

ABSTRACT: A stem cell's decision to self-renew or differentiate is thought to critically depend on signaling cues provided by its environment. It is unclear whether stem cells have the intrinsic capacity to control their responsiveness to environmental signals that can be fluctuating and noisy. Using a novel single-cell microRNA activity reporter, we show that miR-142 is bimodally expressed in embryonic stem cells, creating two states indistinguishable by pluripotency markers. A combination of modeling and quantitative experimental data revealed that mESCs switch stochastically between the two miR-142 states. We find that cells with high miR-142 expression are irresponsive to differentiation signals while cells with low miR-142 expression can respond to differentiation cues. We elucidate the molecul

SUBMITTER: Sladitschek HL 

PROVIDER: S-EPMC4704488 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature