Quantitative analysis of surface plasma membrane proteins of primary and metastatic melanoma cells.

ABSTRACT: Plasma membrane proteins play critical roles in cell-to-cell recognition, signal transduction and material transport. Because of their accessibility, membrane proteins constitute the major targets for protein-based drugs. Here, we described an approach, which included stable isotope labeling by amino acids in cell culture (SILAC), cell surface biotinylation, affinity peptide purification and LC-MS/MS for the identification and quantification of cell surface membrane proteins. We applied the strategy for the quantitative analysis of membrane proteins expressed by a pair of human melanoma cell lines, WM-115 and WM-266-4, which were derived initially from the primary and metastatic tumor sites of the same individual. We were able to identify more than 100 membrane and membrane-associated prot

SUBMITTER: Qiu H 

PROVIDER: S-EPMC4704867 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature