Measurement of lentiviral vector titre and copy number by cross-species duplex quantitative PCR.

ABSTRACT: Lentiviruses are the vectors of choice for many preclinical studies and clinical applications of gene therapy. Accurate measurement of biological vector titre before treatment is a prerequisite for vector dosing, and the calculation of vector integration sites per cell after treatment is as critical to the characterisation of modified cell products as it is to long-term follow-up and the assessment of risk and therapeutic efficiency in patients. These analyses are typically based on quantitative real-time PCR (qPCR), but as yet compromise accuracy and comparability between laboratories and experimental systems, the former by using separate simplex reactions for the detection of endogene and lentiviral sequences and the latter by designing different PCR assays for analyses in human cells an

SUBMITTER: Christodoulou I 

PROVIDER: S-EPMC4705430 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature