ROTS: reproducible RNA-seq biomarker detector-prognostic markers for clear cell renal cell cancer.

ABSTRACT: Recent comprehensive assessments of RNA-seq technology support its utility in quantifying gene expression in various samples. The next step of rigorously quantifying differences between sample groups, however, still lacks well-defined best practices. Although a number of advanced statistical methods have been developed, several studies demonstrate that their performance depends strongly on the data under analysis, which compromises practical utility in real biomedical studies. As a solution, we propose to use a data-adaptive procedure that selects an optimal statistic capable of maximizing reproducibility of detections. After demonstrating its improved sensitivity and specificity in a controlled spike-in study, the utility of the procedure is confirmed in a real biomedical study by identif

SUBMITTER: Seyednasrollah F 

PROVIDER: S-EPMC4705679 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature