Profiling olfactory stem cells from living patients identifies miRNAs relevant for autism pathophysiology.

ABSTRACT:

Background

Autism spectrum disorders (ASD) are a group of neurodevelopmental disorders caused by the interaction between genetic vulnerability and environmental factors. MicroRNAs (miRNAs) are key posttranscriptional regulators involved in multiple aspects of brain development and function. Previous studies have investigated miRNAs expression in ASD using non-neural cells like lymphoblastoid cell lines (LCL) or postmortem tissues. However, the relevance of LCLs is questionable in the context of a neurodevelopmental disorder, and the impact of the cause of death and/or post-death handling of tissue likely contributes to the variations observed between studies on brain samples.

Methods

miRNA profiling using TLDA high-throughput real-time qPCR was performed on miRNAs extracted

SUBMITTER: Nguyen LS 

PROVIDER: S-EPMC4705753 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature