Genome-wide binding analysis of the transcriptional regulator TrmBL1 in Pyrococcus furiosus.

ABSTRACT:

Background

Several in vitro studies document the function of the transcriptional regulator TrmBL1 of Pyrococcus furiosus. These data indicate that the protein can act as repressor or activator and is mainly involved in transcriptional control of sugar uptake and in the switch between glycolysis and gluconeogenesis. The aim of this study was to complement the in vitro data with an in vivo analysis using ChIP-seq to explore the genome-wide binding profile of TrmBL1 under glycolytic and gluconeogenic growth conditions.

Results

The ChIP-seq analysis revealed under gluconeogenic growth conditions 28 TrmBL1 binding sites where the TGM is located upstream of coding regions and no binding sites under glycolytic conditions. The experimental confirmation of the binding sites using qPC

SUBMITTER: Reichelt R 

PROVIDER: S-EPMC4706686 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature