Conformational Selection in a Protein-Protein Interaction Revealed by Dynamic Pathway Analysis.

ABSTRACT: Molecular recognition plays a central role in biology, and protein dynamics has been acknowledged to be important in this process. However, it is highly debated whether conformational changes happen before ligand binding to produce a binding-competent state (conformational selection) or are caused in response to ligand binding (induced fit). Proposals for both mechanisms in protein/protein recognition have been primarily based on structural arguments. However, the distinction between them is a question of the probabilities of going via these two opposing pathways. Here, we present a direct demonstration of exclusive conformational selection in protein/protein recognition by measuring the flux for rhodopsin kinase binding to its regulator recoverin, an important molecular recognition in the

SUBMITTER: Chakrabarti KS 

PROVIDER: S-EPMC4706811 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature