Prolonged Mitosis of Neural Progenitors Alters Cell Fate in the Developing Brain.

ABSTRACT: Embryonic neocortical development depends on balanced production of progenitors and neurons. Genetic mutations disrupting progenitor mitosis frequently impair neurogenesis; however, the link between altered mitosis and cell fate remains poorly understood. Here we demonstrate that prolonged mitosis of radial glial progenitors directly alters neuronal fate specification and progeny viability. Live imaging of progenitors from a neurogenesis mutant, Magoh(+/-), reveals that mitotic delay significantly correlates with preferential production of neurons instead of progenitors, as well as apoptotic progeny. Independently, two pharmacological approaches reveal a causal relationship between mitotic delay and progeny fate. As mitotic duration increases, progenitors produce substantially more apoptot

SUBMITTER: Pilaz LJ 

PROVIDER: S-EPMC4706996 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature