Preexisting smooth muscle cells contribute to neointimal cell repopulation at an incidence varying widely among individual lesions.

ABSTRACT:

Background

With the diverse origin of neointimal cells, previous studies have documented differences of neointimal cell lineage composition across models, but the animal-to-animal difference has not attracted much attention, although the cellular heterogeneity may impact neointimal growth and its response to therapeutic interventions.

Methods

R26R(+);Myh11-CreER(+), and R26R(+);Scl-CreER(+) mice were used to attach LacZ tags to the preexisting smooth muscle cells (SMCs) and endothelial cells (ECs), respectively. Neointimal lesions were created via complete ligation of the common carotid artery (CCA) and transluminal injury to the femoral artery (FA).

Results

LacZ-tagged SMCs were physically relocated from media to neointima and changed to a dedifferentiated phenotype

SUBMITTER: Yang P 

PROVIDER: S-EPMC4707076 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature