Changes in the enhancer landscape during early placental development uncover a trophoblast invasion gene-enhancer network.

ABSTRACT:

Introduction

Trophoblast invasion establishes adequate blood flow between mother and fetus in early placental development. However, little is known about the cis-regulatory mechanisms underlying this important process. We aimed to identify enhancer elements that are active during trophoblast invasion, and build a trophoblast invasion gene-enhancer network.

Methods

We carried out ChIP-Seq for an enhancer-associated mark (H3k27Ac) at two time points during early placental development in mouse. One time point when invasion is at its peak (e7.5) and another time point shortly afterwards (e9.5). We use computational analysis to identify putative enhancers, as well as the transcription factor binding sites within them, that are specific to the time point of trophoblast invasion.

SUBMITTER: Tuteja G 

PROVIDER: S-EPMC4707081 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature