Quantification of heterotypic granule fusion in human neutrophils by imaging flow cytometry.

ABSTRACT: Human neutrophils are filled with intracellular storage organelles, called granules and secretory vesicles, which differ in their content of soluble matrix proteins and membrane-bound molecules. To date, at least four distinct granule/vesicle subsets have been identified. These organelles may secrete their content extracellularly following mobilization to and fusion with the plasma membrane, but some of them may also fuse with internal membrane-enclosed organelles, typically a plasma membrane-derived phagosome. There are also instances where different granules appear to fuse with one another, a process that would enable mixing of their matrix and membrane components. Such granule fusion enables e.g., myeloperoxidase-processing of intragranular oxygen radicals, a key event in the formation

SUBMITTER: Bjornsdottir H 

PROVIDER: S-EPMC4707292 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature