Assembly, Assessment, and Availability of De novo Generated Eukaryotic Transcriptomes.

ABSTRACT: De novo assembly of a complete transcriptome without the need for a guiding reference genome is attractive, particularly where the cost and complexity of generating a eukaryote genome is prohibitive. The transcriptome should not however be seen as just a quick and cheap alternative to building a complete genome. Transcriptomics allows the understanding and comparison of spatial and temporal samples within an organism, and allows surveying of multiple individuals or closely related species. De novo assembly in theory allows the building of a complete transcriptome without any prior knowledge of the genome. It also allows the discovery of alternate splice forms of coding RNAs and also non-coding RNAs, which are often missed by proteomic approaches, or are incompletely annotated in genome stu

SUBMITTER: Moreton J 

PROVIDER: S-EPMC4707302 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature