A Multiplexed System for Quantitative Comparisons of Chromatin Landscapes.

ABSTRACT: Genome-wide profiling of histone modifications can provide systematic insight into the regulatory elements and programs engaged in a given cell type. However, conventional chromatin immunoprecipitation and sequencing (ChIP-seq) does not capture quantitative information on histone modification levels, requires large amounts of starting material, and involves tedious processing of each individual sample. Here, we address these limitations with a technology that leverages DNA barcoding to profile chromatin quantitatively and in multiplexed format. We concurrently map relative levels of multiple histone modifications across multiple samples, each comprising as few as a thousand cells. We demonstrate the technology by monitoring dynamic changes following inhibition of p300, EZH2, or KDM5, by li

SUBMITTER: van Galen P 

PROVIDER: S-EPMC4707994 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature