Hydrogel Pore-Size Modulation for Enhanced Single-Cell Western Blotting.

ABSTRACT: Pore-gradient microgel arrays enable thousands of parallel high-resolution single-cell protein electrophoresis separations for targets accross a wide molecular mass (25-289 kDa), yet within 1 mm separation distances. Dual crosslinked hydrogels facilitate gel-pore expansion after electrophoresis for efficient and uniform immunoprobing. The photopatterned, light-activated, and acid-expandable hydrogel underpins single-cell protein analysis, here for oncoprotein-related signaling in human breast biopsy.

SUBMITTER: Duncombe TA 

PROVIDER: S-EPMC4708057 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature