Evaluating the use of HILIC in large-scale, multi dimensional proteomics: Horses for courses?

ABSTRACT: Despite many recent advances in instrumentation, the sheer complexity of biological samples remains a major challenge in large-scale proteomics experiments, reflecting both the large number of protein isoforms and the wide dynamic range of their expression levels. However, while the dynamic range of expression levels for different components of the proteome is estimated to be ∼10^7-8, the equivalent dynamic range of LC-MS is currently limited to ∼10⁶. Sample pre-fractionation has therefore become routinely used in large-scale proteomics to reduce sample complexity during MS analysis and thus alleviate the problem of ion suppression and undersampling. There is currently a wide range of chromatographic techniques that can be applied as a first dimension separation. Here,

SUBMITTER: Bensaddek D 

PROVIDER: S-EPMC4708065 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature