SOX9 maintains reserve stem cells and preserves radioresistance in mouse small intestine.

ABSTRACT:

Background & aims

Reserve intestinal stem cells (rISCs) are quiescent/slowly cycling under homeostatic conditions, allowing for their identification with label-retention assays. rISCs mediate epithelial regeneration after tissue damage by converting to actively proliferating stem cells (aISCs) that self renew and demonstrate multipotency, which are defining properties of stem cells. Little is known about the genetic mechanisms that regulate the production and maintenance of rISCs. High expression levels of the transcription factor Sox9 (Sox9(high)) are associated with rISCs. This study investigates the role of SOX9 in regulating the rISC state.

Methods

We used fluorescence-activated cell sorting to isolate cells defined as aISCs (Lgr5(high)) and rISCs (Sox9(high)) from Lgr5(

SUBMITTER: Roche KC 

PROVIDER: S-EPMC4709179 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature