Functional properties of flagellin as a stimulator of innate immunity.

ABSTRACT: We report the development of a well-defined flagellin-based nanoparticle stimulator and also provide a new mechanism of action model explaining how flagellin-triggered innate immunity has evolved to favor localized rather than potentially debilitating systemic immune stimulation. Cell-free protein synthesis (CFPS) was used to facilitate mutational analysis and precisely orientated display of flagellin on Hepatitis B core (HBc) protein virus-like particles (VLPs). The need for product stability and an understanding of mechanism of action motivated investigations indicating that the D0 domain of flagellin is sensitive to amino acid sequence independent hydrolysis - apparently due to the need for structural flexibility during natural flagellin polymerization. When D0-stabilized flagellin was

SUBMITTER: Lu Y 

PROVIDER: S-EPMC4709591 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature