Whole-genome sequencing reveals transmission of vancomycin-resistant Enterococcus faecium in a healthcare network.

ABSTRACT:

Background

Bacterial whole-genome sequencing (WGS) has the potential to identify reservoirs of multidrug-resistant organisms and transmission of these pathogens across healthcare networks. We used WGS to define transmission of vancomycin-resistant enterococci (VRE) within a long-term care facility (LTCF), and between this and an acute hospital in the United Kingdom (UK).

Methods

A longitudinal prospective observational study of faecal VRE carriage was conducted in a LTCF in Cambridge, UK. Stool samples were collected at recruitment, and then repeatedly until the end of the study period, discharge or death. Selective culture media were used to isolate VRE, which were subsequently sequenced and analysed. We also analysed the genomes of 45 Enterococcus faecium bloodstream isola

SUBMITTER: Brodrick HJ 

PROVIDER: S-EPMC4709893 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature