Development of molecular approach based on PCR assay for detection of histamine producing bacteria.

ABSTRACT: Histamine fish poisoning becomes highly concern not only in public health but also economic aspect. Histamine is produced from histidine in fish muscles by bacterial decarboxylase enzyme. Several techniques have been developed to determine the level of histamine in fish and their products but the effective method for detecting histamine producing bacteria is still required. This study was attempted to detect histamine producing bacteria by newly developed PCR condition. Histamine producing bacteria were isolated from scombroid fish and determined the ability to produce histamine of isolated bacteria by biochemical and TLC assays. PCR method was developed to target the histidine decarboxylase gene (hdc). The result showed that fifteen histamine producing bacterial isolates and three standar

SUBMITTER: Wongsariya K 

PROVIDER: S-EPMC4711418 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature