EpiTools: An Open-Source Image Analysis Toolkit for Quantifying Epithelial Growth Dynamics.

ABSTRACT: Epithelia grow and undergo extensive rearrangements to achieve their final size and shape. Imaging the dynamics of tissue growth and morphogenesis is now possible with advances in time-lapse microscopy, but a true understanding of their complexities is limited by automated image analysis tools to extract quantitative data. To overcome such limitations, we have designed a new open-source image analysis toolkit called EpiTools. It provides user-friendly graphical user interfaces for accurately segmenting and tracking the contours of cell membrane signals obtained from 4D confocal imaging. It is designed for a broad audience, especially biologists with no computer-science background. Quantitative data extraction is integrated into a larger bioimaging platform, Icy, to increase the visibility

SUBMITTER: Heller D 

PROVIDER: S-EPMC4712040 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature