Identification of sirtuin 5 inhibitors by ultrafast microchip electrophoresis using nanoliter volume samples.

ABSTRACT: Sirtuin 5 (SIRT5) is a member of the sirtuin family of protein deacylases that catalyzes removal of post-translational modifications, such as succinyl and malonyl moieties, on lysine residues. In light of SIRT5's roles in regulating metabolism, and its reported oncogenic functions, SIRT5 modulators would be valuable tools for basic biological research and perhaps clinically. Several fluorescence assays for sirtuin modulators have been developed; however, the use of fluorogenic substrates has the potential to cause false positive results due to interactions of engineered substrates with enzyme or test compounds. Therefore, development of high-throughput screening (HTS) assays based on other methods is valuable. In this study, we report the development of a SIRT5 assay using microchip electr

SUBMITTER: Guetschow ED 

PROVIDER: S-EPMC4712073 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature