A comprehensive benchmarking study of protocols and sequencing platforms for 16S rRNA community profiling.

ABSTRACT:

Background

In the last 5 years, the rapid pace of innovations and improvements in sequencing technologies has completely changed the landscape of metagenomic and metagenetic experiments. Therefore, it is critical to benchmark the various methodologies for interrogating the composition of microbial communities, so that we can assess their strengths and limitations. The most common phylogenetic marker for microbial community diversity studies is the 16S ribosomal RNA gene and in the last 10 years the field has moved from sequencing a small number of amplicons and samples to more complex studies where thousands of samples and multiple different gene regions are interrogated.

Results

We assembled 2 synthetic communities with an even (EM) and uneven (UM) distribution of archaeal

SUBMITTER: D'Amore R 

PROVIDER: S-EPMC4712552 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature