Isolation and characterization of Bradykinin potentiating peptides from Agkistrodon bilineatus venom.

ABSTRACT:

Background

Snake venom is a source of many pharmacologically important molecules. Agkistrodon bilineatus commonly known as Cantil, is spread over Central America particularly in Mexico and Costa Rica. From the venom of Agkistrodon bilineatus we have isolated and characterised six hypotensive peptides, and two bradykinin inhibitor peptides. The IC-50 value of four synthesized peptides was studied, towards angiotensin converting enzyme, in order to study the structure-function relationship of these peptides.

Results

The purification of the peptides was carried out by size exclusion chromatography, followed by reverse phase chromatography. Sequences of all peptides were determined applying MALDI-TOF/TOF mass spectrometry. These hypotensive peptides bear homology to bradykinin p

SUBMITTER: Munawar A 

PROVIDER: S-EPMC4712559 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature