Pateamine A-sensitive ribosome profiling reveals the scope of translation in mouse embryonic stem cells.

ABSTRACT:

Background

Open reading frames are common in long noncoding RNAs (lncRNAs) and 5'UTRs of protein coding transcripts (uORFs). The question of whether those ORFs are translated was recently addressed by several groups using ribosome profiling. Most of those studies concluded that certain lncRNAs and uORFs are translated, essentially based on computational analysis of ribosome footprints. However, major discrepancies remain on the scope of translation and the translational status of individual ORFs. In consequence, further criteria are required to reliably identify translated ORFs from ribosome profiling data.

Results

We examined the effect of the translation inhibitors pateamine A, harringtonine and puromycin on murine ES cell ribosome footprints. We found that pateamine A, a

SUBMITTER: Popa A 

PROVIDER: S-EPMC4712605 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature