Sequencing of lariat termini in S. cerevisiae reveals 5' splice sites, branch points, and novel splicing events.

ABSTRACT: Pre-mRNA splicing is a central step in the shaping of the eukaryotic transcriptome and in the regulation of gene expression. Yet, due to a focus on fully processed mRNA, common approaches for defining pre-mRNA splicing genome-wide are suboptimal-especially with respect to defining the branch point sequence, a key cis-element that initiates the chemistry of splicing. Here, we report a complementary intron-centered approach designed to more efficiently, simply, and directly define splicing events genome-wide. Specifically, we developed a method distinguished by deep sequencing of lariat intron termini (LIT-seq). In a test of LIT-seq using the budding yeast Saccharomyces cerevisiae, we not only successfully captured the majority of annotated, expressed splicing events but also uncovered 45 no

SUBMITTER: Qin D 

PROVIDER: S-EPMC4712674 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature