PAF Complex Plays Novel Subunit-Specific Roles in Alternative Cleavage and Polyadenylation.

ABSTRACT: The PAF complex (Paf1C) has been shown to regulate chromatin modifications, gene transcription, and RNA polymerase II (PolII) elongation. Here, we provide the first genome-wide profiles for the distribution of the entire complex in mammalian cells using chromatin immunoprecipitation and high throughput sequencing. We show that Paf1C is recruited not only to promoters and gene bodies, but also to regions downstream of cleavage/polyadenylation (pA) sites at 3' ends, a profile that sharply contrasted with the yeast complex. Remarkably, we identified novel, subunit-specific links between Paf1C and regulation of alternative cleavage and polyadenylation (APA) and upstream antisense transcription using RNAi coupled with deep sequencing of the 3' ends of transcripts. Moreover, we found that deplet

SUBMITTER: Yang Y 

PROVIDER: S-EPMC4713055 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature