Genome-wide alterations in hippocampal 5-hydroxymethylcytosine links plasticity genes to acute stress.

ABSTRACT: Environmental stress is among the most important contributors to increased susceptibility to develop psychiatric disorders, including anxiety and post-traumatic stress disorder. While even acute stress alters gene expression, the molecular mechanisms underlying these changes remain largely unknown. 5-hydroxymethylcytosine (5hmC) is a novel environmentally sensitive DNA modification that is highly enriched in post-mitotic neurons and is associated with active transcription of neuronal genes. Recently, we found a hippocampal increase of 5hmC in the glucocorticoid receptor gene (Nr3c1) following acute stress, warranting a deeper investigation of stress-related 5hmC levels. Here we used an established chemical labeling and affinity purification method coupled with high-throughput sequencing te

SUBMITTER: Li S 

PROVIDER: S-EPMC4713261 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature