A Comparison of Methods To Enhance Protein Detection of Lipoproteins by Mass Spectrometry.

ABSTRACT: We sought to develop a new method to more efficiently analyze lipid-bound proteins by mass spectrometry using a combination of a lipid removal agent (LRA) that selectively targets lipid-bound proteins and a mass spectrometry compatible detergent, anionic acid labile surfactant (AALS), that is capable of eluting proteins off the LRA. This method was compared to established methods that use the lipid removal agent alone and straight proteomic analysis of human plasma after organic solvent delipidation (OSD). Plasma from healthy individuals was separated by gel filtration chromatography and prepared for mass spectrometry analysis by each of the described methods. The addition of AALS to LRA increased the overall number of proteins detected in both the high and low density lipoprotein size ran

SUBMITTER: Heink A 

PROVIDER: S-EPMC4714602 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature