Deconvolution method for specific and nonspecific binding of ligand to multiprotein complex by native mass spectrometry.

ABSTRACT: In native mass spectrometry, it has been difficult to discriminate between specific bindings of a ligand to a multiprotein complex target from the nonspecific interactions. Here, we present a deconvolution model that consists of two levels of data reduction. At the first level, the apparent association binding constants are extracted from the measured intensities of the target/ligand complexes by varying ligand concentration. At the second level, two functional forms representing the specific and nonspecific binding events are fit to the apparent binding constants obtained from the first level of modeling. Using this approach, we found that a power-law distribution described nonspecific binding of α-amanitin to yeast RNA polymerase II. Moreover, treating the concentration of the multiprote

SUBMITTER: Guan S 

PROVIDER: S-EPMC4714924 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature