High-Resolution Genomic Profiling of Disseminated Tumor Cells in Prostate Cancer.

ABSTRACT: Circulating tumor cells and disseminated tumor cells (DTCs) are of great interest because they provide a minimally invasive window for assessing aspects of cancer biology, including tumor heterogeneity, a means to discover biomarkers of disease behavior, and a way to identify and prioritize therapeutic targets in the emerging era of precision oncology. However, the rarity of circulating tumor cells and DTCs poses a substantial challenge to the consistent success in analyzing their molecular features, including genomic aberrations. Herein, we describe optimized and robust methods to reproducibly detect genomic copy number alterations in samples of 2 to 40 cells after whole-genome amplification with the use of a high-resolution single-nuclear polymorphism-array platform and refined computati

SUBMITTER: Wu Y 

PROVIDER: S-EPMC4715223 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature