Autoantibody signatures defined by serological proteome analysis in sera from patients with cholangiocarcinoma.

ABSTRACT:

Background

The challenging diagnosis and poor prognosis of cholangiocarcinoma require the determination of biomarkers. Autoantibodies could be used in the clinic as diagnostic markers for the early detection of tumours. By proteomic approaches, several autoantibodies were proposed as potential markers. We tried in this study, to perform a serological proteome analysis, using various antigenic substrates, including tumours and human liver.

Methods

Sera from patients (n = 13) and healthy donors (n = 10) were probed on immunoblots performed using 2-dimensionally separated proteins from cholangiocarcinoma cell lines (CCLP1 and CCSW1), from the liver of healthy subject and interestingly, from tumour and adjacent non-tumour liver tissues from five patients with cholangiocarcinoma

SUBMITTER: Mustafa MZ 

PROVIDER: S-EPMC4715332 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature