Whole-Genome Sequencing of a Single Viral Species from a Highly Heterogeneous Sample.

ABSTRACT: Metagenomic studies suggest that only a small fraction of the viruses that exist in nature have been identified and studied. Characterization of unknown viral genomes is hindered by the many genomes populating any virus sample. A new method is reported that integrates drop-based microfluidics and computational analysis to enable the purification of any single viral species from a complex mixed virus sample and the retrieval of complete genome sequences. By using this platform, the genome sequence of a 5243 bp dsDNA virus that was spiked into wastewater was retrieved with greater than 96% sequence coverage and more than 99.8% sequence identity. This method holds great potential for virus discovery since it allows enrichment and sequencing of previously undescribed viruses as well as known v

SUBMITTER: Han HS 

PROVIDER: S-EPMC4715630 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature