Microarray profiling of progesterone-regulated endometrial genes during the rhesus monkey secretory phase.

ABSTRACT:

Background

In the endometrium the steroid hormone progesterone (P), acting through its nuclear receptors, regulates the expression of specific target genes and gene networks required for endometrial maturation. Proper endometrial maturation is considered a requirement for embryo implantation. Endometrial receptivity is a complex process that is spatially and temporally restricted and the identity of genes that regulate receptivity has been pursued by a number of investigators.

Methods

In this study we have used high density oligonucleotide microarrays to screen for changes in mRNA transcript levels between normal proliferative and adequate secretory phases in Rhesus monkey artificial menstrual cycles. Biotinylated cRNA was prepared from day 13 and days 21-23 of the reproduct

SUBMITTER: Ace CI 

PROVIDER: S-EPMC471571 | biostudies-literature | 2004 Jul

REPOSITORIES: biostudies-literature