Protein microarrays identify disease-specific anti-cytokine autoantibody profiles in the landscape of immunodeficiency.

ABSTRACT:

Background

Anti-cytokine autoantibodies (ACAAs) are pathogenic in a handful of rare immunodeficiencies. However, the prevalence and significance of other ACAAs across immunodeficiencies have not yet been described.

Objective

We profiled ACAAs in a diverse cohort of serum samples from patients with immunodeficiency and assessed the sensitivity and specificity of protein microarrays for ACAA identification and discovery.

Methods

Highly multiplexed protein microarrays were designed and fabricated. Blinded serum samples from a cohort of 58 immunodeficiency patients and healthy control subjects were used to probe microarrays. Unsupervised hierarchical clustering was used to identify clusters of reactivity, and after unblinding, significance analysis of microarrays was used

SUBMITTER: Rosenberg JM 

PROVIDER: S-EPMC4715746 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature