ECM Signaling Regulates Collective Cellular Dynamics to Control Pancreas Branching Morphogenesis.

ABSTRACT: During pancreas development, epithelial buds undergo branching morphogenesis to form an exocrine and endocrine gland. Proper morphogenesis is necessary for correct lineage allocation of pancreatic progenitors; however, the cellular events underlying pancreas morphogenesis are unknown. Here, we employed time-lapse microscopy and fluorescent labeling of cells to analyze cell behaviors associated with pancreas morphogenesis. We observed that outer bud cells adjacent to the basement membrane are pleomorphic and rearrange frequently; additionally, they largely remain in the outer cell compartment even after mitosis. These cell behaviors and pancreas branching depend on cell contacts with the basement membrane, which induce actomyosin cytoskeleton remodeling via integrin-mediated activation of F

SUBMITTER: Shih HP 

PROVIDER: S-EPMC4715768 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature