Sequence-function correlations and dynamics of ERG isoforms. ERG8 is the black sheep of the family.

ABSTRACT: The transcription factor ERG is known to have divergent roles. On one hand, it acts as differentiation factor of endothelial cells. On the other hand, it has pathological roles in various cancers. Genomic analyses of the ERG gene show that it gives rise to several isoforms. However, functional differences between these isoforms, representing potential reasons for distinct effects in diverse cell types have not been addressed in detail so far. We set out to investigate the major protein isoforms and found that ERG8 contains a unique C-terminus. This isoform, when expressed as GFP-fusion protein, localized mainly to the cytosol, whereas the other major isoforms (ERG1-4) were predominantly nuclear. Using site directed mutagenesis and laser scanning microscopy of live cells, we could identify

SUBMITTER: Hoesel B 

PROVIDER: S-EPMC4716293 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature