Genes encoding Pir51, Beclin 1, RbAp48 and aldolase b are up or down-regulated in human primary hepatocellular carcinoma.

ABSTRACT:

Aim

To reveal new tumor markers and target genes from differentially expressed genes of primary tumor samples using cDNA microarray.

Methods

The (33 )P labeled cDNAs were synthesized by reverse transcription of message RNA from the liver cancerous tissue and adjacent non-cancerous liver tissue from the same patient and used to hybridize to LifeGrid 1.0 cDNA microarray blot containing 8400 known and unique human cDNA gene targets, and an expression profile of genes was produced in one paired human liver tumor tissue. After a global analysis of gene expression of 8400 genes, we selected some genes to confirm the differential expression using Northern blot and RT-PCR.

Results

Parallel analysis of the hybridized signals enabled us to get an expression profile of genes in

SUBMITTER: Song H 

PROVIDER: S-EPMC4716970 | biostudies-literature | 2004 Feb

REPOSITORIES: biostudies-literature