New mutation points in 23S rRNA gene associated with Helicobacter pylori resistance to clarithromycin in northeast China.

ABSTRACT:

Aim

To investigate the resistance rate of Helicobacter pylori (H pylori ) to clarithromycin, metronidazole, amoxicillin and tetracycline to guide clinical practice, and to study the mechanism of H pylori resistant to clarithromycin.

Methods

Thirty H pylori strains were isolated from the mucosa of peptic ulcer, gastric tumor and chronic gastritis patients, then the minimal inhibitory concentration (MIC) to clarithromycin, metronidazole, amoxicillin and tetracycline was evaluated by E-test method. The sequence analysis of PCR fragments was conducted in 23S rRNA gene of H pylori resistant to clarithromycin to get the resistance mechanism of the bacteria.

Results

Among 30 H pylori strains, 7 cases were resistant to clarithromycin, 12 to metronidazole, 2 to tetracycline an

SUBMITTER: Hao Q 

PROVIDER: S-EPMC4717104 | biostudies-literature | 2004 Apr

REPOSITORIES: biostudies-literature