Uptake of Marasmius oreades agglutinin disrupts integrin-dependent cell adhesion.

ABSTRACT:

Background

Fruiting body lectins have been proposed to act as effector proteins in the defense of fungi against parasites and predators. The Marasmius oreades agglutinin (MOA) is a lectin from the fairy ring mushroom with specificity for Galα1-3Gal containing carbohydrates. This lectin is composed of an N-terminal carbohydrate-binding domain and a C-terminal dimerization domain. The dimerization domain of MOA shows in addition calcium-dependent cysteine protease activity, similar to the calpain family.

Methods

Cell detachment assay, cell viability assay, immunofluorescence, live cell imaging and Western blot using MDCKII cell line.

Results

In this study, we demonstrate in MDCKII cells that after internalization, MOA protease activity induces profound physiological cel

SUBMITTER: Juillot S 

PROVIDER: S-EPMC4717121 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature