Global translational impacts of the loss of the tRNA modification t⁶A in yeast.

ABSTRACT: The universal tRNA modification t⁶A is found at position 37 of nearly all tRNAs decoding ANN codons. The absence of t⁶A₃₇ leads to severe growth defects in baker's yeast, phenotypes similar to those caused by defects in mcm⁵s²U₃₄ synthesis. Mutants in mcm⁵s²U₃₄ can be suppressed by overexpression of tRNA^Lys_UUU, but we show t⁶A phenotypes could not be suppressed by expressing any individual ANN decoding tRNA, and t⁶A and mcm⁵s²U are not determinants for each other's formation. Our results suggest that t⁶A deficiency, like mcm⁵s²U deficiency, leads to protein folding defects, and show that the abs

SUBMITTER: Thiaville PC 

PROVIDER: S-EPMC4717488 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature