TMEM45A Is Dispensable for Epidermal Morphogenesis, Keratinization and Barrier Formation.

ABSTRACT: TMEM45A gene encodes an initially uncharacterized predicted transmembrane protein. We previously showed that this gene is highly expressed in keratinocytes where its expression correlates with keratinization, suggesting a role in normal epidermal physiology. To test this hypothesis, we generated TMEM45A knockout mice and found that these mice develop without any evident phenotype. The morphology of the epidermis assessed by histology and by labelling differentiation markers in immunofluorescence was not altered. Toluidine blue permeability assay showed that the epidermal barrier develops normally during embryonic development. We also showed that depletion of TMEM45A in human keratinocytes does not alter their potential to form in vitro 3D-reconstructed epidermis. Indeed, epidermis with nor

SUBMITTER: Hayez A 

PROVIDER: S-EPMC4718520 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature