Development of Bipotent Cardiac/Skeletal Myogenic Progenitors from MESP1+ Mesoderm.

ABSTRACT: The branchiomeric skeletal muscles co-evolved with new chambers of the heart to enable predatory feeding in chordates. These co-evolved tissues develop from a common population in anterior splanchnic mesoderm, referred to as cardiopharyngeal mesoderm (CPM). The regulation and development of CPM are poorly understood. We describe an embryonic stem cell-based system in which MESP1 drives a PDGFRA+ population with dual cardiac and skeletal muscle differentiation potential, and gene expression resembling CPM. Using this system, we investigate the regulation of these bipotent progenitors, and find that cardiac specification is governed by an antagonistic TGFβ-BMP axis, while skeletal muscle specification is enhanced by Rho kinase inhibition. We define transcriptional signatures of the first com

SUBMITTER: Chan SS 

PROVIDER: S-EPMC4719188 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature