Expression and Genetic Activation of Cyclic Di-GMP-Specific Phosphodiesterases in Escherichia coli.

ABSTRACT:

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Intracellular levels of the bacterial second messenger cyclic di-GMP (c-di-GMP) are controlled by antagonistic activities of diguanylate cyclases and phosphodiesterases. The phosphodiesterase PdeH was identified as a key regulator of motility in Escherichia coli, while deletions of any of the other 12 genes encoding potential phosphodiesterases did not interfere with motility. To analyze the roles of E. coli phosphodiesterases, we demonstrated that most of these proteins are expressed under laboratory conditions. We next isolated suppressor mutations in six phosphodiesterase genes, which reinstate motility in the absence of PdeH by reducing cellular levels of c-di-GMP. Expression of all mutant alleles also led to a reduction of biofilm formation. Thus, all of these prote

SUBMITTER: Reinders A 

PROVIDER: S-EPMC4719445 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature