Evidence for a biphasic mode of respiratory syncytial virus transmission in permissive HEp2 cell monolayers.

ABSTRACT:

Background

During respiratory syncytial virus (RSV) infection filamentous virus particles are formed on the cell surface. Although the virus infectivity remains cell-associated, low levels of cell-free virus is detected during advanced infection. It is currently unclear if this cell-free virus infectivity is due to a low-efficiency specific cell-release mechanism, or if it arises due to mechanical breakage following virus-induced cell damage at the advanced stage of infection. Understanding the origin of this cell-free virus is a prerequisite for understanding the mechanism of RSV transmission in permissive cells. In this study we describe a detailed examination of RSV transmission in permissive HEp2 cell monolayers.

Methods

HEp2 cell monolayers were infected with RSV using

SUBMITTER: Huong TN 

PROVIDER: S-EPMC4719537 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature