Estimating the success of enzyme bioprospecting through metagenomics: current status and future trends.

ABSTRACT: Recent reports have suggested that the establishment of industrially relevant enzyme collections from environmental genomes has become a routine procedure. Across the studies assessed, a mean number of approximately 44 active clones were obtained in an average size of approximately 53,000 clones tested using naïve screening protocols. This number could be significantly increased in shorter times when novel metagenome enzyme sequences obtained by direct sequencing are selected and subjected to high-throughput expression for subsequent production and characterization. The pre-screening of clone libraries by naïve screens followed by the pyrosequencing of the inserts allowed for a 106-fold increase in the success rate of identifying genes encoding enzymes of interest. However, a much longer t

SUBMITTER: Ferrer M 

PROVIDER: S-EPMC4720405 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature