UTR introns, antisense RNA and differentially spliced transcripts between Plasmodium yoelii subspecies.

ABSTRACT:

Background

The rodent malaria parasite Plasmodium yoelii is an important animal model for studying host-parasite interaction and molecular basis of malaria pathogenesis. Although a draft genome of P. yoelii yoelii YM is available, and RNA sequencing (RNA-seq) data for several rodent malaria species (RMP) were reported recently, variations in coding regions and structure of mRNA transcript are likely present between different parasite strains or subspecies. Sequencing of cDNA libraries from additional parasite strains/subspecies will help improve the gene models and genome annotation.

Methods

Here two directional cDNA libraries from mixed blood stages of a subspecies of P. yoelii (P. y. nigeriensis NSM) with or without mefloquine (MQ) treatment were sequenced, and the sequenc

SUBMITTER: Li J 

PROVIDER: S-EPMC4721144 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature