Novel Immortal Cell Lines Support Cellular Heterogeneity in the Human Annulus Fibrosus.

ABSTRACT:

Introduction

Loss of annulus fibrosus (AF) integrity predisposes to disc herniation and is associated with IVD degeneration. Successful implementation of biomedical intervention therapy requires in-depth knowledge of IVD cell biology. We recently generated unique clonal human nucleus pulposus (NP) cell lines. Recurring functional cellular phenotypes from independent donors provided pivotal evidence for cell heterogeneity in the mature human NP. In this study we aimed to generate and characterize immortal cell lines for the human AF from matched donors.

Methods

Non-degenerate healthy disc material was obtained as surplus surgical material. AF cells were immortalized by simian virus Large T antigen (SV40LTAg) and human telomerase (hTERT) expression. Early passage cells and imm

SUBMITTER: van den Akker GG 

PROVIDER: S-EPMC4721917 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature