Reprimo as a modulator of cell migration and invasion in the MDA-MB-231 breast cancer cell line.

ABSTRACT:

Background

Reprimo (RPRM), a highly glycosylated protein, is a new downstream effector of p53-induced cell cycle arrest at the G2/M checkpoint, and a putative tumor suppressor gene frequently silenced via methylation of its promoter region in several malignances. The aim of this study was to characterize the epigenetic inactivation and its biological function in BC cell lines.

Methods

The correlation between RPRM methylation and loss of mRNA expression was assessed in six breast cancer cell lines by methylation specific PCR (MSP), 5'-Aza-2'-deoxycytidine treatment and RT-PCR assays. MDA-MB-231 cells were chosen to investigate the phenotypic effect of RPRM in cell proliferation, cell cycle, cell death, cell migration and invasion.

Results

In the cancer methylome system

SUBMITTER: Buchegger K 

PROVIDER: S-EPMC4722741 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature